Dr. S. Rajkumar

Dr. S. Rajkumar
Principal Scientist

Division/RS/Unit

Division of Genomic Resources

Contact

Off.-011-25802802
Email- s.rajkumar(AT)icar.gov.in, rajnbpgr(AT)gmail.com

Qualification

DegreeDisciplineYear
M.ScBotany1996
Ph.D.Botany2001
Post DoctorateMedicinal Plant Biotechnology2003

SPECIALIZATION
Genetics/Cytogenetics

Salient Achievements

  • DNA Barcode for Species Identification:
  • DNA barcoding was generated for wild and cultivated species of Luffa, vegetable Amaranthus species complex, Trichosanthes cucumerina complex, Cucumis melo, Brinjal, Allium and wild Musa.
  • Genetic Diversity and Population Structure Studies:
  • 3115 safflower accessions from the Indian National Gene Bank, including Indian cultivars were assessed for genetic diversity and population structure using 18 polymorphic SSR markers (Agriculture 2023, 13(4), 836; https://doi.org/10.3390/agriculture13040836 ). Similar studies were carried out in Coix lacryma–jobi and Barley.
  • Core Development: Niger: 3524 accessions from the Indian National Gene Bank were agronomically evaluated at different environments to develop the core set for crop.
  • Safflower: SNP markers obtained from GBS method were utilized for development of molecular core set in 5000 accessions from the Indian National Gene Bank.
  • Transcriptomics: For the first time, cryobionomics of Banana (Musa AAA cv Borjahaji), a non-model species, was investigated through comprehensive transcript profiling during four stages of cryopreservation (Plants 2023, 12(5), 1165; https://doi.org/10.3390/plants12051165 ). The study has shown that the number of DEGs related to membrane lipid remodelling and fatty acid unsaturation (very-long-chain 3-oxoacyl-CoA synthase), as well as stress responsive transcription activators (GRAS, AP2/ERFs, NAC, bZIP and HSFs families), were significantly expressed differentially in sequential stages of cryopreservation.
  • Molecular cytogenetics and Flow Cytometry:
  • Molecular cytogenetics is utilized in species identification in Vegetable Amaranthus and Cucumber.
  • In wild Musa species flow cytometry technique was utilized for their genome size estimation and ploidy determination, which was complemented with cytogenetic studies (Plants 2023, 12(20), 3605; https://doi.org/10.3390/plants12203605), which helped in establishing the identity of samples conserved at the tissue culture of ICAR-NBPGR.
  • Protocol for Fluorescence in situ hybridization (FISH) was standardized for Musa
  • Chromosome identification and karyotype analysis of Vigna umbellata was carried out using Fluorescence in situ hybridization.
  • DNA Fingerprinting:Developed DNA fingerprinting for varietal differentiation in Sunflower and safflower.
  • Temporal variability:Assessment of temporal variation in diversity of long term conserved Safflower germplasm for developing effective regeneration protocol.

Awards/Honours

Recent Publications Research Papers

  1. Pradeep Ruperao, Prasad Bajaj, Rashmi Yadav, Mahalingam Angamuthu, Rajkumar Subramani, Vandana Rai, Kapil Tiwari, Abhishek Rathore, Kuldeep Singh, Gyanendra Pratap Singh, Ulavappa B. Angadi, Sean Mayes, Parimalan Rangan (2024) Double-digest restriction-associated DNA sequencing-based genotyping and its applications in sesame germplasm management. Plant Genome. 2024; e20447
  2. Rajesh Kumar, J. Radhamani, S. Rajkumar 2024 Exploration of allelic diversity reveals a novel FAD2 (Oleate desaturase) gene in Brassica juncea. Crop Breeding and Applied Biotechnology 24(2): e47702424,
  3. Singh, A. Avantika Maurya, Subramani Rajkumar, Amit Kumar Singh , Rakesh Bhardwaj,Surinder Kumar Kaushik, Sandeep Kumar, Kuldeep Singh, Gyanendra Pratap Singh and Rakesh Singh (2024) Comparative Analysis of Five Amaranthaceae Species Reveals a Large Amount of Repeat Content.  Plants 13(824):1-26.
  4. Maurya, S.  Bhumika Sharma, Kajal Thakur, Suman Rawte, Nirmala Bharti Patel, Rajani Bisen,  Rajkumar, Zenu Jha and M. Sujatha (2023) In vitro double haploid production through anther culture in niger (Guizotia abyssinicaL.F. Cass). In Vitro Cell. Dev.Biol.-Plant 60, 50–66
  5. Nagar A, Gowthami R, Sureja AK, Munshi AD, Verma M, Singh AK, Mallick N, Singh J, Chander S, Shankar M, Pathania P and Rajkumar S. 2023. Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency. Plant Sci. 14:1268726.
  6. Natarajan, R.B.; Pathania, P.; Singh, H.; Agrawal, A.; Subramani, R. 2023. A FlowCytometry-Based Assessment of the Genomic Size and Ploidy Level of Wild Musa Species in India. Plants 2023, 12, 3605. https://doi.org/ 10.3390/plants12203605.
  7. Singh A, Mahato AK, Maurya A, Rajkumar S, Singh AK, Bhardwaj R, Kaushik SK, Kumar S, Gupta V, Singh K and Singh R. 2023. Amaranth Genomic Resource Database: an integrated database resource of Amaranth genes and genomics.  Plant Sci. 14:1203855. doi: 10.3389/fpls.2023.1203855
  8. Pradeep Ruperao, Prasad Bajaj, Rajkumar Subramani, Rashmi Yadav, Vijaya Bhaskar Reddy Lachagari, Sivarama Prasad Lekkala, Abhishek Rathore, Sunil Archak, Ulavappa B Angadi, Rakesh Singh, Kuldeep Singh, Sean Mayes, Parimalan Rangan 2023. A pilot-scale comparison between single and double-digest RAD markers generated using GBS strategy in sesame (Sesamum indicum). Plos one 18 (6), e0286599.
  9. Ankit Saroha, Sunil S Gomashe, Vikender Kaur, Deepa Pal, Shraddha Ujjainwal, J Aravind, Mamta Singh, S Rajkumar, Kuldeep Singh, Ashok Kumar, Dhammaprakash Pandhari Wankhede. 2023. Genetic dissection of thousand-seed weight in linseed (Linum usitatissimum L.) using multi-locus genome-wide association study. Frontiers in Plant Science. 14, 1166728.
  10. Kumar, G.P.; Pathania, P.; Goyal, N.; Gupta, N.; Parimalan, R.; Radhamani, J.; Gomashe, S.S.; Kadirvel, P.; Rajkumar, S. 2023 Genetic Diversity and Population Structure Analysis to Construct a Core Collection from Safflower (Carthamus tinctorius) Germplasm through SSR Markers. Agriculture 2023, 13, 836.
  11. Htwe, C.S.S.; Rajkumar, S.; Pathania, P.; Agrawal, A. 2023. Transcriptome Profiling during Sequential Stages of Cryopreservation in Banana (Musa AAA cv Borjahaji) Shoot Meristem. Plants2023, 12, 1165. https://doi.org/10.3390/plants12051165
  12. Vats, G.; Das, D.; Gupta, R.; Singh, A.; Maurya, A.; Rajkumar, S.; Singh, A.K.; Bharadwaj, R.; Kumar, S.; Kaushik, S.K.; Gupta, V.; Singh, K.; Singh, R. 2023. Validation of Genome-Wide SSR Markers Developed for Genetic Diversity and Population Structure Study in Grain Amaranth (Amaranthus hypochondriacus). Agriculture2023, 13, 431. https://doi.org/10.3390/agriculture13020431.
  13. Bansal, S., Sharma, M.K., Singh, S.  Joshi, P., Pathania, P., Malhotra,  ,  S. Rajkumar,   Misra, P. 2023.  Histological and molecular insights in to in vitro regeneration pattern of Xanthosoma sagittifolium. Sci. Rep 13, 5806 (2023).
  14. Parimalan Rangan, DP Wankhede, Rajkumar Subramani Viswanathan Chinnusamy Surendra K. Malik, Mirza Jaynul Baig, Kuldeep Singh, Robert Henry 2022.
    Evolution of an intermediate C4 photosynthesis in the non-foliar tissues of the Poaceae. Photosynthesis Research 153: 125–134
  15. Ankit Saroha . Deepa Pal . Vikender Kaur . Sandeep Kumar . Arti Bartwal .J. Aravind . J. Radhamani. Rajkumar . Rajesh Kumar . Sunil S. Gomashe .Abhishek Sengupta Dhammaprakash Pandhari Wankhede 2022.  Agro-morphological variability and genetic diversityin linseed (Linum usitatissimum L.) germplasm accessionswith emphasis on flowering and maturity time Genet Resour Crop Evol (2022) 69:315–333.
  16. Anjula Pandey, K. Madhav Rai, Pavan Kumar Malav, Rajkumar 2021. Allium negianum (Amaryllidaceae): a new species under subg. Rhizirideum from Uttarakhand Himalaya, India PhytoKeys 183: 77–93 (2021)
  17. Gowthami,R Neelam Sharma, KK Gangopadhyay, Subramani Rajkumar, Pooja Pathania and Anuradha Agrawal Cryopreservation of Pollen of Abelmoschus Moschatus Medik. Subsp. Moschatus as an Aid to Overcome Asynchronous Flowering for Wide Hybridization with Cultivated Okra [A. Esculentus (l.) Moench] Cryo Letters 42(4): 233-244
  18. Pandey, A. and S. Rajkumar, 2021. A new potential variety of cultivated melon (Cucumis melo L.) from north western India. Genet Resour Crop Evol68: 785–794.
  19. Singh , K. Gupta, V. Tyagi, S. Rajkumar 2020. Plant genetic resources in India: management and utilization. Vavilov Journal of Genetics and Breeding 24(3):306-314
  20. Rani, K., Raghu, B.R., Jha, S.K. Priyanka Agarwal Niharika Mallick  Niranjana J. B. Sharma· A. K. Singh  N. K. Sharma S. Rajkumar S. M. S. Tomar Vinod 2020. A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat. Theor Appl Genet (2020). 133: 2685–2694.
  21. Rohini, M.R., Sankaran, M., Rajkumar, S. K. Prakash A. Gaikwad Chaudhury  S. K. Malik 2020. Morphological characterization and analysis of genetic diversity and population structure in Citrus × jambhiri Lush. using SSR markers. Genet Resour Crop Evol 67, 1259–1275.
  22. Kiran Babu P, J Radhamani, S Rajkumar and RK Tyagi. (2019). Note on Diversity in Legumes and Oilseeds and their Wild Relatives in Eastern Ghats of India: Utilization and Conservation Concerns. Indian J. Plant Genet. Resour. 32(1): 43-53
  23. Singh, Kuldeep, Sandeep Kumar, S. Rajkumar, Mohar Singh, Kavita Gupta. 2019. Plant genetic resources management and pre-breeding in genomics era. Indian J. Genet., 79(1) Suppl. 117-130

Disclaimer

The contents of this page are personal information/views of the staff. NBPGR is neither responsible for the contents or reliability nor endorses the views expressed within them.

Scroll to Top